Graphene energy transfer (GET) can be used to measure the distance between a graphene surface and a fluorescent dye. A new strategy for attaching DNA in a controlled orientation onto graphene now makes it possible to use GET to track the dynamics of DNA and protein–DNA interactions with base-pair resolution.
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Fig. 1: Graphene energy transfer (GET) with vertically oriented DNA enables single-molecule measurements of DNA structure and protein–DNA interactions.
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Acknowledgements
I thank Ryan Fantasia, Amanda Wacker and Delisa Ramos for insightful comments and discussions. I am grateful for support from the Arnold and Mabel Beckman Foundation and from the W.M. Keck Foundation.
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Salk Institute for Biological Studies, La Jolla, CA, USA
Pallav Kosuri
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Kosuri, P. Single-molecule DNA dynamics with graphene energy transfer. Nat Methods (2024). https://doi.org/10.1038/s41592-024-02560-8
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Published:10 December 2024
DOI:https://doi.org/10.1038/s41592-024-02560-8
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