The Reds worked at Carrington before the flight to Prague, in advance of the 17:45 GMT kick-off on Thursday.
Centre-backs Jonny Evans and Victor Lindelof could be available again to increase the options open to head coach Ruben Amorim, as he plays a three-man defence.
The experienced campaigners have been missing, with Evans only appearing once for the new boss, in his opening game at Ipswich Town, and Lindelof's last game coming against PAOK in this competition.