The 44 year old's approach was criticised after the number of his players pulled out from international camps last week entered double digits.
Arsenal’s quadruple dreams were extinguished by a disappointing 2-0 loss to Pep Guardiola’s side at Wembley in the Carabao Cup final 12 days ago.
However, the Gunners are still nine points clear in the Premier League title race and will face Saints at St Mary’s in the last eight of the FA Cup.
Arteta's side will also travel to Sporting Club in Portugal for the opening leg of their Champions League quarter-final matchup next week.
"We have very good relationships and communications with most of the national teams, certainly with Thomas (Tuchel)," said Arteta.
Mikel Arteta's side are gunning for a treble this season (Image: PA)
"We’ve been extremely supportive, and when you have to communicate the state and the moment of every player, we are always honest.
"In the end, it’s a medical decision that has to be made. That was clear what the conclusion was. When a player is fit and available, they have to play.
"I think it makes us so proud that we have that many players in the national team, and the players are desperate to play in the national team.
"We know how important every time they are together and the time they spend in preparation means to them, so we are fully supportive of that."
Arteta was asked how many of the players who did not play for their countries would be available to play against Saints, but refused to answer.
"I will continue the speculation so you can judge it afterwards, if what I say was right or you have to delete and write something else," said Arteta.
"We are in a position now that we are going to make the strongest line-ups and decisions that we possibly can to win every competition.
"We are two or three games away in the FA Cup and we know how important that it is for us. Huge competition, so related to the history of our club.
"We’re going to play a team that is in a really good moment, they have won so many games recently, they’re in a really good moment.
"You know the expectations, the crowd, the environment they are going to create, so we are ready for that, and we want to be at Wembley again."