Thomas Tuchel in England training
Ezri Konsa and Nico O'Reilly missed England training on Monday ahead of a Nations League clash against the Czech Republic.
The two Three Lions stars were not included in team preparations, instead following their own individual programmes.
Ezri Konsa playing for England3
Konsa was not part of England's team training on Monday morning
Nico O'Reilly playing for England3
O'Reilly was also absent, with both stars now doubts for Tuesday's match
Both Konsa and O'Reilly started and played the full 90 minutes of Thomas Tuchel's side's opening defeat to Spain.
O'Reilly provided the assist for Harry Kane's first-half goal before making a costly error as La Roja won 3-2.
The pair are now doubts for England's clash with the Czech Republic on Tuesday evening, which is live on talkSPORT.
Lewis Hall and Jarrad Branthwaite could potentially take their place in the match at the Fortuna Arena in Prague.
Declan Rice, Marcus Rashford, Cole Palmer, Kobbie Mainoo and Tino Livramento have all already pulled out for England.
Now, Konsa and O'Reilly, who were the only players missing from training on Monday, could be carrying injury issues.
After facing the Czech Republic on Tuesday night, they still have another clash against them and against Croatia coming up.
So, losing two starting defenders would be a big blow for Tuchel, with three games still to come in the international break.
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International injury troubles continue
The potential injuries would be a blow for England, but also for Arsenal and Manchester City.
Konsa has become a regular for the Gunners after his move from Aston Villa, with O'Reilly key for Man City.
England squad walking to training3
Konsa and O'Reilly were the only two players to miss training - 22 others took part
Both of their clubs will hope that the issues are nothing serious and that they don't miss any action.
Particularly for Mikel Arteta, as he may have already lost one of his key players during the break.
Kai Havertz could be out for a month after picking up a hamstring injury while playing for Germany last week.
Meanwhile, Manchester United have also been dealt potential blows, with two of their players picking up knocks.
Captain Bruno Fernandes sat out for Portugal against Norway, while Patrick Dorgu has been sent home from Denmark duty after being forced off with an injury against Wales on Sunday.
Liverpool's Cody Gakpo has also returned home early after being substituted early in the Netherlands' game against Serbia.
Alexander Isak left the Sweden camp early too, as Premier League players continue to be hit with problems on the national stage.