Abstract
Targeting drug-resistant Acinetobacter baumannii, two fungal strains, Epicoccum sp. 1-042 and Penicillium sp. 19-115, were identified from 35 fungi isolated from Tibet. Bioassay-guided isolation from Epicoccum sp. 1-042 yielded a novel cyclohexenone, epiconone (1), and parasitenone (2), while patulin (3) was isolated from Penicillium sp. 19-115. Structural elucidation was accomplished through comprehensive spectroscopic analysis and quantum chemistry calculations. The biosynthetic pathways of compounds 1 and 2 were proposed based on bioinformatics analysis. Compounds 1 − 3 exhibited antibacterial activity against carbapenem-resistant Acinetobacter baumannii (CRAB) with MIC values ranging from 4 to 128 μg mL-1.
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Acknowledgements
This research was co-funded by The National Natural Science Foundation of China (82104047) and CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1-028).
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These authors contributed equally: Shanshan Chang, Xinyue Huang.
Authors and Affiliations
CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, People’s Republic of China
Shanshan Chang, Xinyue Huang, Meng Liu, Ning He, Yihong Li, Mengna Luo, Mengyuan Wang & Yunying Xie
School of Food Science and Technology, Jiangnan University, Lihu Road No.1800, Wuxi, 214122, People’s Republic of China
Meng Liu
NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, People’s Republic of China
Yan Li
CAMS Key Laboratory of Antiviral Drug Research, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tiantan xili No.1, Beijing, 100050, People’s Republic of China
Hu Li
Department of Laboratory Medicine, Jiangnan University Medical Center (Wuxi No.2 People’s Hospital), Wuxi, 214000, People’s Republic of China
Xichi Hu
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Shanshan Chang
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Chang, S., Huang, X., Liu, M. et al. Epiconone, one novel cyclohexenone from endophytic fungi Epicoccum sp. 1-042. J Antibiot (2025). https://doi.org/10.1038/s41429-025-00813-y
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Received:29 December 2024
Revised:08 February 2025
Accepted:18 February 2025
Published:07 March 2025
DOI:https://doi.org/10.1038/s41429-025-00813-y
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