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Systematic review and meta‑analysis of acaricidal properties of plant derived products against Ixodid ticks population

Application of plant extracts and plant-based formulations is one of the sustainable methods for the management

of resistant ticks and thus managing the tick-borne diseases. In the present study, we comprehensively analyzed the

research papers based on the acaricidal activity of plant extracts. A meta-analysis was conducted with an aim to highlight

the potential candidates for further research for the development of ecofriendly and sustainable anti-tick herbal

formulations. A total of 52 papers were analyzed up until December 2023, sourced from extensive searches of databases

such as PubMed, Scopus, Science Direct and Google Scholar, focusing on the acaricidal activity of various plants against

tick species. The plants belonging to Annonaceae and Burseraceae families were found significantly effective against

ticks. Amongst the tick species, Rhipicephalus microplus was the highly studied tick species followed by Haemaphysalis

bispinosa and Hyalomma dromedarii. The most responsive tick species to plant extracts were Hyalomma dromedarii and

Ambylomma cajannense. With respect to the parts of the plants used, leaf (45%) was extensively utilized while the extracts

prepared from the resin, stem and fruit peel of the plant showed highest acaricidal activity. The present study highlighted

significant variability in the effectiveness of plant extracts depending on the plant species, plant parts used, type of

extractant, and the tick species tested. These findings emphasize the need to develop a Standard Operating Protocol

(SOP) for evaluating phytocompounds. Future research should prioritize isolating active compounds, elucidating their

mechanisms of action, and optimizing formulations to ensure sustainable and effective tick management strategies.

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