Trans-splicing enables the ligation of exons from different pre-mRNAs into a single transcript. A recent study harnesses this process and develops riboregulators that, upon binding programmable external guide sequences (EGSs), allow customizable, orthogonal and predictable gene circuit construction.
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*Fig. 1: Mechanism and applications of trans-splicing facilitated by EGS.*
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School of Life Sciences, Arizona State University, Tempe, AZ, USA
Samat Bayakhmetov
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA
Mahan Naseri & Xiao Wang
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Samat Bayakhmetov
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2. Mahan Naseri
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Bayakhmetov, S., Naseri, M. & Wang, X. Trans-splicing for gene regulation. Nat Chem Biol (2025). https://doi.org/10.1038/s41589-025-01871-x
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Published:27 March 2025
DOI:https://doi.org/10.1038/s41589-025-01871-x
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