The showpiece game of the PL2 season will take place at the Joie Stadium tomorrow evening, with kick-off scheduled for 7:15pm.
Saints booked their place in the final for the first time by beating Crystal Palace at Selhurst Park, with Derrick Abu scoring an extra-time winner.
Southampton under-21s goalkeeper Josh McNamara.Southampton under-21s goalkeeper Josh McNamara. (Image: Craig Hobbs / Southampton FC) READ: Saints goalkeeper calls for 'heart and togetherness' ahead of national final
Calum McFarlane's side have also knocked out Leicester City with a thrilling 5-3 victory and regular season runners-up Fulham, beating them 5-2 at St Mary's.
Manchester City topped the regular season table and progressed to the final by beating Liverpool, Arsenal, and local rivals Manchester United.
The battle for the second-ever iteration of the PL2 play-off title will crown a new winner of the competition's new format.
Tottenham lifted the trophy last season, beating Sunderland in the final, with neither Saints nor Man City qualifying for the post-season knockout stage.